Screening For α-Amylase Inhibitory Activity Of Crataegus And Rubus Genera In Syria, Isolation And Identification Of The Active Principle

One of the treatment strategies for diabetes mellitus is to inhibit specific digestive enzymes in order reduce carbohydrates absorption intestines. In this study, inhibitory activity toward α-amylase enzyme were determined methanolic extracts leaves two species genus Crataegus; monogyna, and azarolus var aronia, three Rubus; sanctus, hedycarpus, collinus. The collected during spring autumn seasons from different regions across Syrian Arab Republic. extract C.azarolus aronia harvested exhibited strongest compared other with an IC50=103 µg/ml. For a closer look into plant, dry was suspended water extracted (liquid-liquid) using diethyl ether ethyl acetate as extracting solvents. Due promising IC50=96 µg/ml, further fractionated by means preparative HPLC. structure isolated compound that revealed (IC50=84 µg/ml) elucidated nuclear magnetic resonance (NMR), mass spectroscopy (MS) techniques. identified based on MS NMR data be (–)-Epicatechin, biologically active flavan-3-ol